Fig. 4

Binding specificity of selected single aptamers, A1–A6, to (1 → 3)-β-D-glucans (curdlan, barley glucan, and (1 → 3)-β-D-glucans obtained from Candida albicans) and non-(1 → 3)-β-D-glucans (mannan, endotoxin, and dextran). A The binding specificities of selected single aptamers (A1 ~ A6) to (1 → 3)-β-D-glucans were evaluated by enzyme-linked oligonucleotide assay. The absorbance was proportional to affinity between corresponding aptamer-polysaccharide pair, and vice versa. B We used heat maps to describe the binding specificity of the single aptamer A1 A6 to (1 3) -β-D-glucan and non-(1 3) -β-D-glucan. All data were expressed as mean standard deviation, and the experiment was repeated five times