Fig. 7
The quantification of (1 → 3)-β-D-glucans in 198 human serum specimens using aptamer G-quadruplex/hemin self-assembling color system (AGSCS). A A total of 198 serum samples were pipetted into 96-well microplates. Aptamers with G-quadruplex were added together with hemin and tetramethylbenzidine/H2O2. H2SO4 was subsequently added to stop the reaction of the AGSCS, and a microplate reader was used to measure the absorbance at 450 nm. All data are shown as means ± standard deviation, and experiments were performed in triplicates. B Receiver operating characteristics analysis of the sensitivity and specificity of AGSCS. The area under the curve was 0.9122