Table 1 Demographic characteristics of study participants
Patient NO. | Sex | Age | Infection part | Precipitating factor | Classifications of rash | Months from symptom onset to specimen collection | Sample classification | mNGS results | Relative abundance | Sequence number | Culture results | Culture and/or identification day | T-SPOTⓇ results | Histopathology Special stains | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Acid-fast | PAS-D | ||||||||||||||
1 | F | 64 | Left fingers and arm | Fishbone | Pink linear nodule | 10 | Tissue | M. marinum | 28.5% | 2 | M. marinum | 35 | + | + | ND |
Human gammaherpesviru | 100% | 17 | |||||||||||||
2 | F | 68 | Left arm | Soil | Red papule and linear nodule | 5 | Tissue | M. ulcerans | 13.7% | 4 | M. marinum | 18 | + | - | - |
M. marinum | 10.3% | 3 | |||||||||||||
3 | M | 48 | Right hand | Branch of trees | Nodule and cystis | 3 | Paraffin section | Mycobacterium spp. | 15.7% | 225 | ND | ND | ND | ND | ND |
S. aureus | 6.3% | 91 | |||||||||||||
Tissue | ND | ND | ND | - | - | ||||||||||
4 | F | 66 | Right hand | Faucet | Red plaque | 6 | Paraffin section | - | - | - | ND | ND | ND | ND | ND |
Tissue | ND | ND | ND | - | - | - | ND | ||||||||
5 | M | 57 | Right finger and opisthenar | Fishbone | Red plaque | 24 | Tissue | Pluralibacter gergoviae | 92.73% | 689 | Pluralibacter gergoviae | 2 | - | - | - |
M.marinum | 0.27% | 2 | M. marinum | 23 | |||||||||||
M. ulcerans | 0.13% | 1 | |||||||||||||
6 | M | 78 | Right leg | Soil | Giant plaque and swelling | 312 | Paraffin section | Fonsecaea monophora | 78% | 25 | ND | ND | - | ND | ND |
Tissue | ND | ND | ND | Fonsecaea monophora | 14 | ||||||||||
7 | M | 35 | Left knee and hand | Fall damage | Red plaque | 24 | Paraffin section | M. abscessus | 0.14% | 2 | ND | ND | ND | ND | ND |
Tissue | ND | ND | ND | - | - | + | ND | ||||||||
8 | F | 55 | Left leg | UNK | Swelling and ulceration | 2 | Pus | M. abscessus | 30% | 97 | M. abscessus | 25 | - | + | - |
9 | M | 65 | Central face | UNK | Swelling and nodule | 12 | Paraffin section | - | - | - | ND | ND | - | ND | ND |
Tissue | ND | ND | ND | - | - | ND | + | ||||||||
10 | M | 19 | Right forearm | Bruise | Linear nodule | 10 | Tissue | - | - | - | - | - | ND | - | - |
11 | F | 57 | Right midfinger and forearm | Fishbone | Swelling, ulceration and nodules | 8 | Tissue | - | - | - | - | - | + | - | - |
12 | M | 30 | Right index finger | Bruise | Swelling and nodule | 12 | Tissue | M. marinum | 11% | 1 | M. marinum | 32 | - | - | - |
M. ulcerans | 11% | 1 | |||||||||||||
13 | M | 42 | Left inner thigh and the dorsum of left foot | Fishbone | Verrucous plaques | 360 | Tissue | S. aureus | 40.55% | 8194 | M.tuberculosis | 38 | + | - | - |
14 | F | 65 | Right hand and forearm | Branch of trees | Red plaque and nodules | 12 | Tissue | M. gallinarum | 1.07% | 1 | - | - | ND | + | ND |
15 | M | 60 | Left forearm | UNK | Swelling and ulceration | 1 | Tissue | Talaromyces marneffei | 59.58% | 1349 | Talaromyces marneffei | 7 | ND | ND | ND |
Chryseobacterium gleum | 40.42% | 915 | |||||||||||||
Pus | Talaromyces marneffei | 32.37% | 414 | ND | ND | ND | + | ||||||||
Chryseobacterium gleum | 67.63% | 865 | |||||||||||||
16 | F | 52 | Face | Cosmetic injection | Nodule and cystis | 3 | Tissue | - | - | - | - | - | ND | + | + |
17 | F | 69 | Right inner ankle | Nails | ulcers | 4 | Tissue | C.parapsilosis | 98.1% | 893,307 | C.parapsilosis | 6 | - | - | - |
18 | M | 18 | Left upper limb | Branch of trees | Swelling, nodule | 2 | Tissue | M.marinum | 4.8% | 65 | - | - | + | - | - |
Pseudomonas aeruginosa | 7% | 754 | |||||||||||||
19 | M | 29 | Right hand | Fishbone | Erythema, Swelling | 5 | Tissue | M.marinum | 3.8% | 5 | Positive | 12 | + | + | ND |
20 | F | 55 | Right middle finger | UNK | erythema | 15 | Tissue | C.parapsilosis | 64.71% | 11 | - | - | + | ND | |
21 | M | 50 | Right wrist | UNK | erythema | 12 | Tissue | M.marinum | 0.45% | 2 | - | - | + | - | - |
M.ulcerans | 0.45% | 2 | |||||||||||||
22 | M | 72 | Left dorsal and medial ankle | Nails | Erythema, papules, nodules, ulcers | 3 | Tissue | M.houstonense | 33.59% | 43 | Positive** | 11 | - | - | - |
23 | F | 54 | Right forearm | UNK | papules | 60 | Tissue | Sporothrix globosa | 17% | 3 | Sporothrix schenckii | 32 | - | - | - |
M.restricta | 43% | 5 | |||||||||||||
Torque teno virus | 38% | 7 | |||||||||||||
24 | F | 69 | Right hand | UNK | plaque | 36 | Tissue | M.marinum | 2.5% | 3 | Lactobacillus plantarum | 3 | - | - | - |
Pneumocystis jirovecii | 80% | 5 | |||||||||||||
25 | M | 52 | Right elbow | UNK | erythema | 12 | Tissue | M.marinum | 0.8% | 1 | Positive | 19 | ND | - | - |
Streptococcus dysgalactiae | 1.1% | 5 | |||||||||||||
26 | M | 65 | Left thumb and left forearm | UNK | nodule | 3 | Tissue | M.marinum | 0.7% | 9 | Positive | 36 | + | - | - |
C.parapsilosis | 66.9% | 60 | |||||||||||||
M.restricta | 21.4% | 9 | |||||||||||||
Klebsiella pneumoniae | 5.9% | 172 | |||||||||||||
Enterobacter cloacae complex | 1.3% | 70 | |||||||||||||