Comparing the diagnostic value of targeted with metagenomic next-generation sequencing in immunocompromised patients with lower respiratory tract infection

Table 2 Performance characteristics of targeted NGS in 88 clinic samples

Patients characteristics (n = 88)	tNGS positive	tNGS negative	Sensitivity (%)	Specificity (%)	PPV (%)	NPV (%)	Agreement (%)	Kappa value
Positive by culture	37	2	94.87	10.20	45.68	71.43	47.73	0.06
Negative by culture	44	5
Positive by CMT	52	3	94.55	15.15	65.00	62.50	64.77	0.06
Negative by CMT	28	5
Positive by composite reference standard	76	8	90.48	50.00	97.43	20.00	88.64	0.21
Negative by composite reference standard	2	2
Positive by mNGS	81	3	96.43	75.00	98.78	50.00	94.32	0.58
Negative by mNGS	2	2

Abbreviations CMT: Conventional microbiological test; tNGS: Targeted Next-generation sequencing; mNGS: Metagenomic next-generation sequencing; PPV: Positive predictive value; NPV: Negative predictive value

ISSN: 1476-0711