Porin expression in clinical isolates of Klebsiella pneumoniae: a comparison of SDS-PAGE and MALDI-TOF/MS and limitations of whole genome sequencing analysis

Elías-López, Cristina; Muñoz-Rosa, Montserrat; Guzmán-Puche, Julia; Pérez-Nadales, Elena; Chicano-Galvez, Eduardo; Martínez-Martínez, Luis

doi:10.1186/s12941-024-00761-9

Annals of Clinical Microbiology and Antimicrobials

Table 2 Expression of porins grown in nutrient broth, mutations in the corresponding genes and molecular weight

From: Porin expression in clinical isolates of Klebsiella pneumoniae: a comparison of SDS-PAGE and MALDI-TOF/MS and limitations of whole genome sequencing analysis

ID_Isolate	OmpA				OmpK35				OmpK36
ID_Isolate	Protein expression	WGS	MW WGS (kDa)	MW MALDI- TOF/MS (kDa)	Protein expression	WGS	MW WGS (kDa)	MW MALDI- TOF/MS (kDa)	Protein expression	WGS	MW WGS (kDa)	MW MALDI- TOF/MS (kDa)
ATCC 13883	+	Reference	35.96	35.75	+	Reference	37.23	37.03	+	Reference	38.69	38.55
CHURS-5-1534	+	NM	35.96	35.65	+	NM	37.23	36.94	+	NM	38.69	38.45
CHURS-9-1075	+	NM	35.96	35.77	+	NM	37.23	37.04	+	M	37.97	37.77
CHURS-170652	+	NM	35.96	35.76	+	NM	37.23	37.04	+	M	38.12	37.93
CHURS-170744	+	NM	35.96	35.77	+	NM	37.23	37.07	+	M	37.96	37.79
CHURS-170943	+	NM	35.96	35.77	+	NM	37.23	37.03	+	M	38.12	37.93
CHURS-171503	+	NM	35.96	35.75	+	NM	37.23	37.04	+	M	37.94	37.77
CHURS-171703	+	NM	35.96	35.76	+	NM	37.23	37.05	+	M	37.96	37.78
CHURS-174873	+	NM	35.96	35.75	+	NM	37.23	37.06	+	M	37.89	37.70
CHURS-175802	+	NM	35.96	35.77	+	NM	37.23	37.02	+	NM	38.69	38.58
ATCC 700603	+	M	35.96	35.75	-	M	37.25	X	+	M	37.54	37.36
RHURS-0113	+	NM	35.96	35.76	-	NM	37.23	X	+	M	37.98	37.81
RHURS-0114	+	NM	35.96	35.76	-	NM	37.23	X	+	M	37.98	37.79
RHURS-0115	+	NM	35.96	35.76	-	NM	37.23	X	-	P	37.98	X
RHURS-0039	+	P	36.13	35.97	-	Stop-codon-67	8.01	X	+	M	37.96	37.80
RHURS-0308	+	NM	35.96	35.76	-	Stop-codon-67	8.01	X	+	M	38.13	37.95
CHURS-000952	+	NM	35.96	35.84	-	Stop-codon-67	8.01	X	+	M	37.96	37.85
CHURS-170942	+	NM	35.96	35.76	-	Stop-codon-67	8.01	X	+	M	38.13	37.94
CHURS-175691	+	NM	35.96	35.76	-	Stop-codon-67	8.01	X	+	M	38.13	37.95
CHURS-180130	+	NM	35.96	35.75	-	Stop-codon-67	8.01	X	+	M	38.13	37.96
CHURS-184677	+	NM	35.96	35.76	-	Stop-codon-67	8.01	X	+	M	37.96	37.77
RHURS-0132	+	NM	35.96	35.89	-	Stop-codon-15	1.57	X	-	Stop-codon-65	7.27	X
CHURS-009-IS	+	NM	35.96	35.87	-	P	37.45	X	+	M	37.96	37.90
CHURS-000694	+	NM	35.96	35.85	-	Stop-codon-15	1.57	X	-	Stop-codon-171	18.65	X
CHURS-000958	+	NM	35.96	35.83	-	Stop-codon-15	1.57	X	+	M	37.96	37.84
CHURS-001262	+	NM	35.96	35.84	-	Stop-codon-15	1.57	X	+	M	37.96	37.89
CHURS-181073	+	NM	35.96	35.83	-	Stop-codon-15	1.57	X	+	M	37.96	37.85
CHURS-183019	+	NM	35.96	35.84	-	Stop-codon-15	1.57	X	+	M	37.96	37.83

Presence (+) / absence (-) of band or peak. NM (no wt mutation). M (multiple mutations). P (point mutation). Stop-codon-x (position of the protein with a stop codon). X (no peaks)

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ISSN: 1476-0711

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