Fig. 2

Estimated maximum likelihood phylogenetic analysis of the four Cefiderocol (FDC) non-susceptible carbapenem-resistant Acinetobacter baumannii isolates. The maximum likelihood was inferred from a core genome alignment of 3,344,425 bp. The phylogeny was estimated with IqTree using the best-fit model of nucleotide substitution HKY + F with 1,000 replicates and fast bootstrapping. The numbers on leaves represent the sample IDs, and bootstrap values are shown on branches. Information regarding the samples was reported: date of isolation, the sequence type (ST), capsular locus (K locus) and lipooligosaccharide outer core (OC locus), the presence (solid squares) or absence of antimicrobial resistance genes, the presence of deletion in pirA gene, the Cefiderocol-non-susceptibility, tested by disk diffusion (according to the last EUCAST guidelines, https://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_14.0_Breakpoint_Tables.pdf) and the minimal inhibitory concentration (MIC) value by the UMIC (Bruker) commercial test