Fig. 10

Representative photomicrographs exhibited Hematoxylin and Eosin (H&E)-stained skin Sect. (2 days) (scale bar, 100 μm, × 100). (A) Microscopic photographs of skin tissues obtained from control and treated groups. Control tissues display epidermal cells necrosis, intense inflammatory cells infiltration and marked edema. Vancomycin- treated group demonstrates inflammatory cells infiltration and congested blood vessel. Black arrow indicates necrosis of epidermal cells, red arrow indicates inflammatory cells, blue arrow indicates edema while yellow arrow indicates congested blood vessel. PSK-treated group (2 hpi) shows focal necrosis of epidermal epithelium and moderate inflammatory cells infiltration. PSK-treated group (2 dpi) shows inflammatory cells infiltration and edema. The statistical analysis of the histopathological scores of necrosis (B) and inflammatory cells infiltration (C). The data expressed as the median and interquartile range (p25-p75). Statistics were carried out by Kruskal Wallis test followed by Dunn test. (D) The antibacterial activity of either PSK or vancomycin after 2 hpi or dpi. *, p < 0.05; **, p < 0.01; ***, p < 0.001