Fig. 2
Kinetic and stability evaluation of Staphylococcus phage PSK. (A) Adsorption curve was obtained by mixing PSK with its host (S. aureus SK1) at MOI of 0.01, then free phages were counted, expressed as percentage and plotted against time (min). (B) One-step growth curve was constructed by mixing PSK and its host stains at final MOI of 0.01, then phage count was monitored over 90 min. (C) Thermal stability of PSK was assessed by incubating it for 1 h at test temperature, then serially diluted, counted and expressed as percentage. (D) pH stability was validated by incubating PSK (1010 PFU/mL suspended in SM buffer at pH 7.5) at different pH values (4–11), then infective PSK were counted and expressed as percentage. Each value represents the mean of three replicates ± standard deviation. A paired Student t test was conducted to compare significance between mean values, with a temperature of 4 °C and pH 7 as references (*, p < 0.05)