Table 2 Virulence factor candidates in the Nocardia cyriacigeorgica according to the Virulence Factor Database (VFDB)

Adherence

clpC

VFG000079

Endopeptidase Clp ATP-binding chain C, is required for adhesion and promoting early escape form the phagosome of macrophages

dnaK

VFG050290

Chaperone protein

eno

VFG005576

Phosphopyruvate hydratase

groEL

VFG012095

Chaperone protein

htpB

VFG001855

60 K heat shock protein HtpB

plr/gapA

VFG005358

Glyceraldehyde-3-phosphate dehydrogenase

tufA

VFG046465

Elongation factor Tu

Invasion

Mce3

VFG010120

Mammalian cell entry family proteins

Mce4

VFG010259

Mammalian cell entry family proteins

Mce5

VFG010296

Mammalian cell entry family proteins

Mce6

VFG010326

Mammalian cell entry family proteins

Mce7

VFG010381

Mammalian cell entry family proteins

Mce8

VFG010393

Mammalian cell entry family proteins

Mce9

VFG010427

Mammalian cell entry family proteins

Stress survival

ahpC

VFG001404

Putative alkylhydroper oxide reductase

clpP

VFG000077

Serine protease involved in proteolysis and is required for growth under stress conditions

katA

VFG037028

Catalase, protects against reactive oxygen species

katG

VFG001396

Catalase peroxidase

sodA

VFG001421

Putative superoxide dismutase, important for intracellular survival and transmission

sodC

VFG001380

Putative superoxide dismutase, important for intracellular survival and transmission

Nutritional/metabolic factor

irtB

VFG024069

ABC transporter ATP-binding protein

carB

VFG047708

Carbamoyl phospate synthase large subunit

ctpV

VFG031419

Copper exporter

dahP

VFG044426

DAHP synthetase

entB

VFG048419

Enterobactin, involved in Iron uptake

fbpC

VFG001811

Secreted antigen 85-C, is an important immunomodulatory component of the cell wall

hemL

VFG013203

Glutamate-1-semialdehyde-2,1-aminomutase

irp5

VFG016174

Yersiniabactin biosynthetic protein irp5

mbtA

VFG001817

Salicyl-AMP ligase + salicyl-S-ArCP synthetase

pchH

VFG001267

Pyochelin

mbtB

VFG001403

Phenyloxazoline synthase

mbtC

VFG001823

Polyketide synthetase

mbtE

VFG001821

Peptide synthetase

mbtF

VFG001820

Peptide synthetase

mbtG

VFG001819

Lysine-N-oxygenase

mbtH

VFG001818

Putative protein

mbtI

VFG009561

Isochorismate synthase

narG

VFG001391

Nitrate reductase alpha subunit

narH

VFG001814

Nitrate reductase beta subunit

narI

VFG001816

Nitrate reductase gamma subunit

narJ

VFG001815

Nitrate reductase delta subunit

Immune modulation

adhD

VFG030297

MymA operon, involved in mycolic acid synthesis

gndA

VFG048830

6-phosphogluconate dehydrogenase

mps1

VFG029717

GPL locus

wcbT

VFG002546

8-amino-7-oxononanoate synthase, involved in antiphagocytosis

ptpA

VFG031511

Protein-tyrosine phosphatase, involved in phagosome arresting

ndk

VFG031473

Nucleoside diphosphate kinase, involved in phagosome arresting

Regulation

devR/dosR

VFG001824

Two component transcriptional regulator, LuxR family

ideR

VFG001406

Positive regulator of oxidative stress responses

relA

VFG001826

Probable GTP pyrophosphokinase, associated with stationary phase adaptation and long-term survival

phoP

VFG001386

Possible two component system response transcriptional positive regulator

Effector delivery system

eccB3

VFG022047

Type VII secretion system protein

eccC4

VFG022232

Type VII secretion system protein

espR

VFG021908

Type VII secretion system protein

ricA

VFG045340

Rab2 interacting conserved protein A

Motility

flmH

VFG038840

Polar flagella

Exotoxin

pgi

VFG038132

Endotoxin, glucose-6-phosphate isomerase

rtxB

VFG038918

RTX toxin transporter

rtxE

VFG007023

RTX toxin transporter